The latest development moves USA's Jennifer Brady, Ajla Tomljanovic of Australia, and Russia's Svetlana Kuznetsova into the main draw.
For the qualifiers, Kazakhstan's Zarina Diyas has also pulled out due to a yet to be confirmed injury. Russia's duo of Liudmila Samsonova and Natalia Vikhlyantseva, as well as Belgium's' Ysaline Bonaventure, have now been placed in the qualifiers.
Tournament Director Saad Al Mohannadi said, "Though we would have loved to see Andreescu and the rest of the stars play at this year's tournament, we, however, understand that their health is more important at this moment. We wish them a speedy recovery and also wish them the best as we look forward to welcoming them to Doha in the future."
Qatar Total Open 2020 is the 18th edition of the annual WTA Premier 5 tournament and this year's event carries a total prize purse of $2,939,695.